FastQCFastQC Report
Thu 4 Sep 2025
SRR25630385_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR25630385_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33181752
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTTGAGGCGCCCTGGGACTGCGTCTATTTTTACTCCTAGTGCTGGT669590.20179464905891648No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT617260.18602393267239173No Hit
CTGCGAGTGATTTAGCACCCGGCTCCCGCGAACGTGAGTTTCGTGGCCGG513710.1548170211144969No Hit
GTTGGAATTGCGATAATTATTGTTGCTGATGTGAAGTAGGCTCGGGTGTC408330.12305860160729307No Hit
CTCATCAGTAAATTGAGACGTAAAGGAATAGTCAGACTACGTCAACAAAG352570.10625418452889408No Hit
GTTTTATGTTGATGATTGTGGTGATAAAGTTAATTGAACCAAGGATGGAG352370.10619391043607343No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348170.10492815448683963No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)